Hygiena - ATP Testing Solution Malta

The Scientific way to prove cleanliness!

The EnSure™ cleaning verification solution consists of a luminometer, ATP test devices and the SureTrend™ dashboard. These three parts work together to help you track and measure your cleaning program across multiple locations. Learn more about each test below.


The ATP test is a process of rapidly measuring actively growing microorganisms through detection of adenosine triphosphate, or ATP.

​Using UltraSnap™ surface ATP tests couldn’t be easier. The three-step process for collecting a sample, activating the device, and mixing the sample is so easy, anyone can do it.


How does ATP testing work?

Hygiena™ ATP testing devices contain a natural enzyme found in fireflies. This enzyme produces a simple bioluminescent (light-producing) reaction when it comes into contact with ATP (Adenosine triphosphate, the energy-carrying molecule found in the cells of all living things). Using bioluminescence technology, the SystemSURE™ Plus luminometer can measure extremely low levels of ATP collected with testing devices.

Measuring the amount of bioluminescence from an ATP reaction provides an excellent indication of surface cleanliness because the quantity of light generated by the reaction is directly proportional to the amount of ATP present in the sample. The bioluminescence reaction is immediate so results can be processed at the testing site in seconds. Results are expressed numerically on the luminometer screen in Relative Light Units (RLU).

Bioluminescence is the result of a biochemical reaction and is the science behind Hygiena™ ATP detection tests. The reaction includes the following elements:

  • Luciferin /Luciferase -naturally occurring in fireflies

  • Adenosine triphosphate (ATP) – the energy molecule of all living organisms

  • Oxygen- a catalyst

Luciferin /Luciferase + ATP + O2 → Light output


The reaction occurs in two steps:

  1.  The substrate combines with ATP and oxygen, which is controlled by the enzyme.

  2. The chemical energy in step 1 excites a specific molecule (the combination of Luciferin and Luciferase). The result is decay which is manifested as photon emission, or light production. The light is simply a byproduct of the chemical reaction and does not depend on light.